This experiment investigated caffeine’s effect on asparagus root development and the absorption of minerals. Previous studies have tested different concentrations of caffeine in a nutrient medium; thus, obtaining data on caffeine’s effect under optimal nutrient conditions becomes necessary. The influence of caffeine on plant yield and seed germination rates changes depending on different concentrations. To solidify the effects of different concentrations, this experiment has been conducted using concentrations of decaffeinated and caffeinated green tea. Given the prolonged germination period of asparagus seedlings, a pre-planting soaking procedure in water was done for 24 hours to enhance germination. After soaking the seedlings, they were placed in rockwool to germinate. Data was collected weekly on the root growth, and the fresh weight was collected after the experiment. By week 4, the root growth of 1% decaffeinated green tea exceeded threefold of what was observed in the control group. After week 4, all groups excluding 1% decaffeinated exhibited less root development than the control group. Concentrations of 2% or concentrations that contain caffeine restrict root growth and fresh weight. The fresh weight of seedlings that received caffeinated treatments is half that of seedlings treated with 1% decaffeinated solution. Notably, the sprouting ratio was most influenced by green tea concentrations rather than caffeine content, with 2% decaffeinated and caffeinated concentrations exhibiting the greatest sprouting ratio. With high root length and full sprouting ratio, asparagus seedlings treated with 1% decaffeinated green tea indicated the inefficiency of caffeine on improving the germination rate of asparagus seedlings.

References

• Q., Wang, N., Liu, H., Li, Z., Lu, L., & Wang, C. (2020). The bioactive compounds and biological functions of Asparagus officinalis L.–A review. Journal of Functional Foods, 65, 103727.
• Parihar, S., & Sharma, D. (2021). A brief overview on Asparagus racemous. IJRAR, 8(4), 96-108.
• Negi, J., Singh, P., Joshi, G., Rawat, M., & Bisht, V. (2010). Chemical constituents of Asparagus. Pharmacognosy Reviews, 4(8), 215.
• Namita, P., Mukesh, R., & Vijay, K. J. (2012). Camellia sinensis (green tea): a review. Global journal of pharmacology, 6(2), 52-59..
• Perumalla, A. V. S., & Hettiarachchy, N. S. (2011). Green tea and grape seed extracts— Potential applications in food safety and quality. Food Research International, 44(4), 827- 839.
• Pérez-Burillo, S., Navajas-Porras, B., López-Maldonado, A., Hinojosa-Nogueira, D., Pastoriza, S., & Rufián-Henares, J. Á. (2021). Green tea and its relation to the human gut microbiome. Molecules, 26(13), 3907.
• Sinclair, C. J., & Geiger, J. D. (2000). Caffeine use in sports. A pharmacological review. J Sports Med Phys Fitness, 40(1), 71-79.
• Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A., & Feeley, M. (2003). Effects of caffeine on human health. Food Additives & Contaminants, 20(1), 1-30.
• Koo, Y. M., Heo, A. Y., & Choi, H. W. (2020). Salicylic Acid as a Safe Plant Protector and Growth Regulator. The plant pathology journal, 36(1), 1–10.
• Kim, Y. S., & Sano, H. (2008). Pathogen resistance of transgenic tobacco plants producing caffeine. Phytochemistry, 69(4), 882-888.
• Muratova, S. A., Papikhin, R. V., & Khoroshkova, Y. V. (2020). The effect of caffeine in a nutrient medium on rhizogenesis of the Rubus genus plants.In BIO Web of Conferences (Vol. 23, p. 03013). EDP Sciences.
• Yamane, K., Kono, M., Fukunaga, T., Iwai, K., Sekine, R., Watanabe, Y.,Iijima, M. (2014). Field evaluation of coffee grounds application for crop growth enhancement, weed control, and soil improvement. Plant Production Science, 17(1), 93-102.